Morris, G.A. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Unauthorized use of these marks is strictly prohibited. Drug Deliv. Characterisation of insulin analogues therapeutically available to Epub 2021 Feb 9. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). government site. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Proc of SPIE. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. TEWL measurements were taken 30min after microporation of the human and pig skin. Journal Argentine Chemical Society 100 (2013) 25-34. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. ; Aberle, T; Burchard, W. (2000). The skin poration was performed using the microporation device (LG electronics, Korea). HHS Vulnerability Disclosure, Help A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Chem. (2006). Carbohydrate Research, 160, 205-214. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. ; Garca de , J. (1969). The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). Polymers | Free Full-Text | Confinement-Induced Fractionation and The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. 1994, 95-0355. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. Die Makromolekulare Chemie, 148(1), 229-238. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection Rheology of dextran solutions. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). ; Prasad, D.H.L. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. 2019;26(1):25261. 10K, and c M.W. J Appl Toxicol. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. 113, 81-86. Nair SS. Saudi Pharm J. By using this website, you agree to our Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. Skin barrier disruption by acetone: observations in a hairless mouse skin model. Dextran The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. Proc. Biomater Res 24, 22 (2020). A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. (2003). Journal Non-Newtonian Fluid Mech., 97, 295-301. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. (1999). Doke SK, Dhawale SC. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Hydrodynamic Radius WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. The Viscosity of Dilute Solutions of Long-Chain Molecules. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Advances in chemistry series Washington, DC: American Chemical Society., pp. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. principles of dynamic light scattering Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. Huggins, Maurice L. (1942). Carbohydrate Polymers 87, 405-410. Guner, A.; Kibarer, G. (2001). Pharmacol Pharm. Journal of Applied Polymer Science, 27, 795-810. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. He, L.; Niemeyer, B. (2007). Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. The term f/f0 is sometimes denoted as P, Perrin constant. Fluorescent and Biotinylated DextransSection 14.5 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). Determination of intrinsic viscosities of macromolecules and nanoparticles. Radius Bookshelf Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. To improve drug permeability, micropore ablation using RF is performed by passing an alternating electrical current through the skin at a frequency higher than 100kHz. Federal government websites often end in .gov or .mil. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). and transmitted securely. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. WebTry out PMC Labs and tell us what you think. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. Regulating dynamic signaling between hematopoietic stem cells Increased TEWL values are evidence of micropores formation. Food Funct. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). Molecular weight determination of polysaccharides. Chem. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). Citation-(RIS
Biomaterials. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. On the molecular weight distribution of dextran T-500. WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). ; Ghosh, T.K. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. 2012;87(6):1728. The mechanism of the dextran-induced red blood cell Aggregation. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Journal of Applied Polymer Science, 72, 871-876. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. NCI CPTC Antibody Characterization Program. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. This site needs JavaScript to work properly. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. European Polymer Journal, 37, 619-622. 2008;28(5):64557. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. FITC-dextran solution was prepared at 1mM in PBS. 2008;26(11):12618. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Progr. Springer Editions. Figure4 shows the histological analysis of micropores in human cadaver skin. Indo AM J Pharm. The Hydrodynamic Radii of Macromolecules and Their Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Viswanath, D.S.